Document Details
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Document Type |
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Article In Journal |
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Document Title |
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A Comparison and Integration of MiSeq and MinION Platforms for Sequencing Single Source and Mixed Mitochondrial Genomes A Comparison and Integration of MiSeq and MinION Platforms for Sequencing Single Source and Mixed Mitochondrial Genomes |
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Document Language |
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English |
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Abstract |
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Single source and multiple donor (mixed) samples of human mitochondrial DNA were analyzed
and compared using the MinION and the MiSeq platforms. A generalized variant
detection strategy was employed to provide a cursory framework for evaluating the reliability
and accuracy of mitochondrial sequences produced by the MinION. The feasibility of longread
phasing was investigated to establish its efficacy in quantitatively distinguishing and
deconvolving individuals in a mixture. Finally, a proof-of-concept was demonstrated by integrating
both platforms in a hybrid assembly that leverages solely mixture data to accurately
reconstruct full mitochondrial genomes. |
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ISSN |
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1932-6203 |
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Journal Name |
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PloS one |
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Volume |
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11 |
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Issue Number |
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12 |
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Publishing Year |
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1437 AH
2016 AD |
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Article Type |
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Article |
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Added Date |
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Tuesday, July 18, 2017 |
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Researchers
| Michael R. Lindberg | Lindberg, Michael R. | Researcher | Doctorate | |
| Sarah E Schmedes | Schmedes, Sarah E | Researcher | Doctorate | |
| F Curtis Hewitt | Hewitt, F Curtis | Researcher | Doctorate | |
| Jamie L. Haas | Haas, Jamie L. | Researcher | Doctorate | |
| Krista L. Ternus | Ternus, Krista L | Researcher | Doctorate | |
| Dana R. Kadavy | Kadavy, Dana R | Researcher | Doctorate | |
| Bruce Budowle | Budowle, Bruce | Researcher | Doctorate | |
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